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Evidence Guide: MSL975056 - Perform advanced histological techniques

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL975056 - Perform advanced histological techniques

What evidence can you provide to prove your understanding of each of the following citeria?

Prepare specialised tissues

  1. Prepare agar plugs for cytological or minute specimens in accordance with workplace procedures
  2. Prepare blood clot for processing in accordance with workplace procedures
  3. Prepare friable /tiny tissue in sponges and/or wrapped to ensure tissue integrity and prevent loss during processing in accordance with workplace procedures
  4. Decalcify bone in accordance with workplace procedures
  5. Identify and prioritise specimens prior to embedding
  6. Select appropriately sized mould
  7. Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy
  8. Embed tissue with embedding orientation markings
  9. Confirm number of tissues is as expected and record any discrepancies
  10. Inspect blocks, reject and re-embed items that do not meet quality control standards
  11. Prepare and adjust all external parts of a microtome and associated equipment to accommodate requirements of tissue batch
  12. Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts
  13. Apply microtomy techniques to trim blocks to full face without significant loss of tissue
  14. Cut and pick up thin tissue sections according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing
  15. Inspect sections and reject any that do not meet quality control standards
  16. Identify, troubleshoot and resolve common section quality control issues
  17. Apply quality control procedures to troubleshoot discrepancies between request-sheet and sections on slides
Prepare agar plugs for cytological or minute specimens in accordance with workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare blood clot for processing in accordance with workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare friable /tiny tissue in sponges and/or wrapped to ensure tissue integrity and prevent loss during processing in accordance with workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Decalcify bone in accordance with workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify and prioritise specimens prior to embedding

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select appropriately sized mould

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue with embedding orientation markings

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Confirm number of tissues is as expected and record any discrepancies

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect blocks, reject and re-embed items that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare and adjust all external parts of a microtome and associated equipment to accommodate requirements of tissue batch

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply microtomy techniques to trim blocks to full face without significant loss of tissue

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut and pick up thin tissue sections according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect sections and reject any that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify, troubleshoot and resolve common section quality control issues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply quality control procedures to troubleshoot discrepancies between request-sheet and sections on slides

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge